Abstarct: Abstract Seed burnout is an abiotic stress that can cause cell damage, delayed seedling emergence, reduced ability to withstand stresses, and ultimately poor seedling establishment. In the present study, the germination percentage, germination rate, and changes in soybean mextabolic profile were investigated using GC-MS in response to seed burnout stress and gallic acid pretreatment. The experiment was conducted as a factorial experiment in a randomized complete block design with burnout factors including two levels of stress and no stress and gallic acid pretreatment at zero levels and 25 and 50 mg/L. The results showed that seed burnout significantly reduced the percentage and rate of seed germination. Pretreatment of depleted seeds with concentrations of 25 and 50 mg/L of gallic acid improved the percentage and rate of seed germination by 25 and 22%, respectively, compared to no pretreatment (control). The mextabolic pattern of the resulting seedling extract showed that 73 compounds from the sugars, alcohols, fatty acids, amino acids, and organic compounds were measured jointly in all replicates at different levels of depletion stress and gallic acid pretreatment. The use and non-use of gallic acid pretreatment under seed depletion conditions resulted in a diversity of mextabolic content. Depletion stress affected the mextabolic pathway of some amino acids such as glycine, serine, and threonine in a decreasing manner, and in contrast, the mextabolic pathway of carbohydrates, especially galactose, fructose, and mannose, in an increasing manner. Gallic acid pretreatment in depleted seeds, in addition to significantly affecting the mextabolic pathway of sugars, The biosynthesis of some fatty acids, mextabolic pathways of amino acids such as alanine, aspartate and glutamate and specifically the antioxidant pathway of ascorbate mextabolism were significantly affected under conditions of pretreatment with 25 mg/L gallic acid. Thus, in stress conditions, the plant pursues energy supply through changes in the content of mextabolites, and gallic acid pretreatment, especially at a concentration of 25 mg/L, caused positive changes in the mextabolic pathways of carbohydrates, amino acids and some fatty acids, while strengthening this process by inhibiting free radicals and improving defense strategies in conditions of seed deterioration, helping to reduce the consequences of exhaustion and increase seed and seedling vigor.