Abstarct: Abstract Norozak (Salvia leriifolia) is an Iranian species of the genus Salvia of the mint family. This study aimed to investigate the effect of ellagic acid on the growth, enzymatic and physiological characteristics of Salvia at the Faculty of Agriculture, Shahrood University of Technology. The experiment was conducted in a completely randomized design with ellagic acid at concentrations of 0, 50, 100 and 150 mg/L in four-week-old in vitro seedlings of Salvia. Each treatment had five replicates, with three seedlings in a 250 cc culture bottle as one replicate. The results showed that the effect of different concentrations of ellagic acid on the number of leaves, leaf length and root diameter had no significant effect. With increasing ellagic acid concentration, leaf width and root length decreased compared to the control, but stem length increased. The highest variable fluorescence was recorded at a concentration of 150 mg/L of ellagic acid. Also, with increasing ellagic acid concentration, the amount of proline in in vitro seedlings of Norozak plantlet decreased, so that the highest amount of proline was observed in seedlings without ellagic acid treatment (in vitro seedlings of control) and 50 mg/L of ellagic acid. Catalase enzyme activity was not different from the control up to a concentration of 100 mg/L, but it increased sharply at 150 mg/L. The lowest activity of guaiacol peroxidase enzyme was observed in the control treatment and a concentration of 150 mg/L. Also, the amount of malondialdehyde decreased under ellagic acid treatment. baxsed on the results of the present study, the use of ellagic acid treatment in in vitro seedlings of Norozak can activate the oxidative defense system, so further studies are recommended to deal with biotic and abiotic stresses.